ELISA TransDetect® Cell LIVE|DEAD Viability|Cytotoxicity Detection

Product Details The TransDetect® Cell LIVE/DEAD Viability/Cytotoxicity Detection Kit provides a simple, high-specificity and low-toxicity way to distinguish between live and dead cells by combining Calcein-AM and Propidium iodide (PI). Quantification or sorting can be performed by flow cytometry. Calcein-AM is a low-cytotoxicity non-fluorescent dye, which easily permeates intact live cells. In live cells, the Calcein-AM is converted to green fluorescent calcein with excitation and emission wavelengths of 490/515 nm by intracellµLar esterase digestion. PI is excluded by intact plasma membrane of live cells. It is a DNA-binding fluorescent dye with excitation and emission wavelengths of 535/617 nm， producing a bright red fluorescence in dead cells with broken membrane. • Higher specificity. • More simplicity. • Lower cytotoxicity. Storage at -20℃ in the dark for up to one year. Shipping Dry ice (-70℃) Product Contents Component FC301-01 (100 rxns) Calcein-AM Solution(1000×) 100 µL PI Solution(1000×) 100 µL