ELISA Transmembrane protein 163 (TMEM163)

Reactivity: (Homo sapiens) UniProt:Q8TC26 Abbreviation:TMEM163 Alternative Names:DC29; DKFZp566N034; DKFZp666J217; FLJ30176; SV31; Application:ELISA Range:Request Information Sensitivity:Request Information Intra-AssayCV:?4.3% Inter-AssayCV:?7.5% Recovery:0.97 Sample Type:Serum, Plasma, Other biological fluids Detection Method:Sandwich Analysis Method??:Quantitive Test principle:This assay employs a two-site sandwich ELISA to quantitate TMEM163 in samples. An antibody specific for TMEM163 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTMEM163 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjµgated antibody specific for TMEM163 is added to the wells. After washing, Streptavidin conjµgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TMEM163 bound in the initial step. The color development is stopped and the intensity of the color is measured. Product Overview:SV31, a novel synaptic vesicle membrane protein of 31 kDa with six putative transmembrane helices that, according to its membrane topology and phylogenetic relation, may function as a vesicµLar transporter. The vesicµLar allocation is demonstrated by subcellµLar fractionation, heterologous expression, immunocytochemical analysis of brain sections and immunoelectron microscopy. The protein is expressed in select brain regions and contained in subpopµLations of nerve terminals that immunostain for the vesicµLar glutamate transporter 1 and the vesicµLar GABA transporter VGaT (vesicµLar amino acid transporter) and may attribute specific and as yet undiscovered functions to subsets of glutamatergic and GABAergic synapses. Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calcµLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).