ELISA Thyroxine-Binding Globulin (TBG)

Reactivity: (Homo sapiens) UniProt:P05543 Abbreviation:SERPINA7 Alternative Names:RP1-82J11.2; TBG; OTTHUMP00000023779|alpha-1 antiproteinase; antitrypsin|serine (or cysteine) proteinase inhibitor; clade A (alpha-1 antiproteinase; antitrypsin); member 7|serine (or cysteine) prote Application:ELISA Range:0.78-50 ng/mL Sensitivity:0.31 ng/mL Intra-AssayCV:?4.1% Inter-AssayCV:?7.3% Recovery:0.99 Sample Type:Serum, Plasma, Other biological fluids Detection Method:Sandwich Analysis Method??:Quantitive Test principle:This assay employs a two-site sandwich ELISA to quantitate SERPINA7 in samples. An antibody specific for SERPINA7 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySERPINA7 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjµgated antibody specific for SERPINA7 is added to the wells. After washing, Streptavidin conjµgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SERPINA7 bound in the initial step. The color development is stopped and the intensity of the color is measured. Product Overview:Thyroxine-binding globµLin (TBG) binds Thyroid hormone in circµLation. It is one of three proteins (along with transthyretin and albumin) responsible for carrying the thyroid hormones thyroxine (T4) and 3,5,3’-triiodothyronine (T3) in the bloodstream. Of these three proteins, TBG has the highest affinity for T4 and T3, but is present in the lowest concentration. Despite its low concentration, TBG carries the majority of T4 in serum. Due to the very low serum concentration of T4 & T3, TBG is rarely more than 25% saturated with its ligand. Unlike transthyretin and albumin, TBG has a single binding site for T4/T3. TBG is syntheQuantityd primarily in the liver as a 54 kDa protein. Genomically, TBG is a serpin, althoµgh it has no inhibitory function like many other members of this class of proteins. Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calcµLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).