ELISA T7 RNAi Transcription

The purified transcript can be used for RNAi experiments mediated by cationic liposome, calcium phosphate coprecipitation, electroporation, DEAE-dextran and microinjection. Kit Composition Components TR102-01 50 rxn TR102-02 100 rxn T7 Enzyme Mix 50 μl 100 μl 10 × Transcription Buffer 50 μl 100 μl 10 × Annealing Buffer 250 μl 500 μl NTP Mix 200 μl 400 μl DNase I 25 μl 50 μl RNase T1 (100 U/μl) 25 μl 50 μl RNase T1 Dilution Buffer 300 μl 600 μl Control Template* 5 μl 10 μl RNase-free H2O RNA Clean Beads 5 mL 2 mL 5 mL 4 mL Storage: Store all the components at -20℃.