ELISA T7 High Efficiency Transcription

Product Details T7 High Efficiency Transcription Kit is designed for in vitro RNA synthesis by T7 RNA Polymerase with supercoiled or linearized DNA templates. More than 50 μg of RNA can be produced from a 20 μl reaction with 1 μg of template added. SyntheQuantityd RNA can be used for in vitro translation, RNase protection assays, RNA splicing, and hybridization assays. Storage at -20°C for one year Shipping Dry ice (-70 ℃) Product Contents Component JT101-01 (25 rxns) T7 Transcription Enzyme Mix 50 μl 5×T7 Transcription Reaction Buffer 100 μl 10 mM NTP Mix 200 μl DNase I (1 unit/μl) 25 μl 500 mM EDTA (pH 8.0) 25 μl RNase-free Water 500 μl Control Transcription Template (0.5 μg/μl) 10 μl