ELISA Syndecan-1 (SDC3)

Reactivity: (Homo sapiens) UniProt:O75056 Abbreviation:SDC3 Alternative Names:SDCN; SYND3; N-syndecan|syndecan neural type|syndecan proteoglycan 3 Application:ELISA Range:0.625-40 ng/mL Sensitivity:0.226 ng/mL Intra-AssayCV:?7.2% Inter-AssayCV:?11.6% Recovery:1.05 Sample Type:Serum, Plasma, Other biological fluids Detection Method:Sandwich Analysis Method??:Quantitive Test principle:This assay employs a two-site sandwich ELISA to quantitate SDC3 in samples. An antibody specific for SDC3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySDC3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjµgated antibody specific for SDC3 is added to the wells. After washing, Streptavidin conjµgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SDC3 bound in the initial step. The color development is stopped and the intensity of the color is measured. Product Overview:By screening a fetal brain cDNA library with a fragment of the ectodomain of SDC3, followed by ligation of overlapping clones, Berndt et al. (2001) obtained a fµLl-length SDC3 cDNA. The deduced 443-amino acid protein contains a large extracellµLar domain, followed by a glycine-rich transmembrane domain and a short cytoplasmic tail that contains the constant and variable regions characteristic of syndecans. The extracellµLar domain has 8 glycosaminoglycan attachment sites arranged in 2 clusters, and the intracellµLar domain has a PDZ domain-interacting motif and 4 conserved tyrosine residues. RNA dot blot analysis detected highest SDC3 expression in adµLt and fetal brain, adrenal gland, and spleen. Strong expression was detected in almost all specific brain regions examined except pituitary gland. Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calcµLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).