ELISA Spleen tyrosine kinase (SYK)

Reactivity: (Homo sapiens) UniProt:P43405 Abbreviation:SYK Alternative Names:DKFZp313N1010; FLJ25043; FLJ37489; OTTHUMP00000021628|OTTHUMP00000021629 Application:ELISA Range:0.312-20 ng/mL Sensitivity:0.118 ng/mL Intra-AssayCV:?4.7% Inter-AssayCV:?8.1% Recovery:0.98 Sample Type:Serum, Plasma, Other biological fluids Detection Method:Sandwich Analysis Method??:Quantitive Test principle:This assay employs a two-site sandwich ELISA to quantitate SYK in samples. An antibody specific for SYK has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySYK present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjµgated antibody specific for SYK is added to the wells. After washing, Streptavidin conjµgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SYK bound in the initial step. The color development is stopped and the intensity of the color is measured. Product Overview:SYKis a member of the Syk family of tyrosine kinases. While Syk and Zap-70 are primarily expressed in hematopoietic tissues, there is expression of Syk in a variety of tissues. Within B and T cells respectively, Syk and Zap-70 transmit signals from the B-Cell receptor and T-Cell receptor. Syk plays a similar role in transmitting signals from a variety of cell surface receptors including CD74, Fc Receptor, and integrins. The role of Syk in epithelial malignancies is controversial. Several authors have sµggested that abnormal Syk function facilitates transformation in Nasopharyngeal carcinoma and head and neck cancer while other authors have sµggested a tumor suppressor role in breast and gastric cancer. Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calcµLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).