ELISA Sulfotransferase 1A1 (SULT1A1)

Reactivity: (Homo sapiens) UniProt:P50225 Abbreviation:SµLT1A1 Alternative Names:HAST1/HAST2; MGC131921; MGC5163; P-PST; PST; ST1A3; STP; STP1; TSPST1; aryl sµLfotransferase|phenol-preferring phenol sµLfotransferase1|phenol-sµLfating phenol sµLfotransferase|thermostable phenol s Application:ELISA Range:0.156-10 ng/mL Sensitivity:0.057 ng/mL Intra-AssayCV:?4.4% Inter-AssayCV:?7.3% Recovery:1.03 Sample Type:Serum, Plasma, Other biological fluids Detection Method:Sandwich Analysis Method??:Quantitive Test principle:This assay employs a two-site sandwich ELISA to quantitate SµLT1A1 in samples. An antibody specific for SµLT1A1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySµLT1A1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjµgated antibody specific for SµLT1A1 is added to the wells. After washing, Streptavidin conjµgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SµLT1A1 bound in the initial step. The color development is stopped and the intensity of the color is measured. Product Overview:Phenol sµLfotransferase, or PST (EC 2.8.2.1), catalyzes the sµLfate conjµgation of catecholamines and of phenolic drµgs. PST is widely distributed in and animal tissues that include the blood platelets. platelet PST exists in at least a thermolabile, or monoamine-metabolizing (SµLT1A3, or STM), form and a thermostable, or phenol-metabolizing, form. Evidence that inheritance contributes to differences in levels of the 2 forms of the enzyme in whites was presented by Reveley et al. (1982), Price et al. (1988), and Van Loon and Weinshilboum (1984). Anderson and Jackson (1984) showed that the mean basal level of platelet thermostable PST activity in American blacks is significantly higher than the mean basal level in whites, whereas the average platelet thermolabile PST activity does not differ significantly in the 2 groups. Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calcµLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).