ELISA Stress-induced-phosphoprotein 1 (STIP1)

Reactivity: (Homo sapiens) UniProt:P31948 Abbreviation:STIP1 Alternative Names:HOP; IEF-SSP-3521; P60; STI1; STI1L; Hsp70/Hsp90-organizing protein|stress-induced-phosphoprotein 1 (Hsp70/Hsp90-organizing protein) Application:ELISA Range:1.56-100 ng/mL Sensitivity:0.63 ng/mL Intra-AssayCV:?4.5% Inter-AssayCV:?7.6% Recovery:1.02 Sample Type:Serum, Plasma, Other biological fluids Detection Method:Sandwich Analysis Method??:Quantitive Test principle:This assay employs a two-site sandwich ELISA to quantitate STIP1 in samples. An antibody specific for STIP1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySTIP1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjµgated antibody specific for STIP1 is added to the wells. After washing, Streptavidin conjµgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of STIP1 bound in the initial step. The color development is stopped and the intensity of the color is measured. Product Overview:Hop is the Hsp70-Hsp90 organizing protein. It functions as a co-chaperone which reversibly links together the protein chaperones Hsp70 and Hsp90.The gene for Hop is located on chromosome 11q13.1 and consists of 14 exons. HOP (the abbreviation stands for Hsp70/Hsp90 Organizing Protein) belongs to the large group of co-chaperones, which regµLate and assist the major chaperones (mainly heat shock proteins). It is one of the best studied co-chaperones of the Hsp70/Hsp90-complex. It was first discovered in yeast and homologues were identified in , mouse, rat, insects, plants, parasites, and virus. The family of these proteins is referred to as STI1 (stress inducible protein) and can be divided into yeast, plant, and animal STI1 (Hop). Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calcµLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).