ELISA Steroidogenic acute regulatory protein, mitochondrial (STAR)

Reactivity: (Homo sapiens) UniProt:P49675 Abbreviation:STAR Alternative Names:STARD1; START domain containing 1|StAR-related lipid transfer (START) domain containing 1|cholesterol trafficker|mitochondrial steroid acute regµLatory protein|steroid acute regµLatory protein|stero Application:ELISA Range:0.156-10 ng/mL Sensitivity:0.064 ng/mL Intra-AssayCV:?4.6% Inter-AssayCV:?7.9% Recovery:0.83 Sample Type:Serum, Plasma, Other biological fluids Detection Method:Sandwich Analysis Method??:Quantitive Test principle:This assay employs a two-site sandwich ELISA to quantitate STAR in samples. An antibody specific for STAR has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySTAR present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjµgated antibody specific for STAR is added to the wells. After washing, Streptavidin conjµgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of STAR bound in the initial step. The color development is stopped and the intensity of the color is measured. Product Overview:The steroidogenic acute regµLatory protein is a transport protein that regµLates cholesterol transfer within the mitochondria, which is the rate-limiting step in the production of steroid hormones. It is primarily present in steroid-producing cells, including theca cells and luteal cells in the ovary, Leydig cells in the testis and cell types in the adrenal cortex. The domain of StAR important for promoting cholesterol transfer is the StAR-related transfer domain (START domain). StAR is the prototypic member of the START domain family of proteins and is thus also known as STARD1 for "START domain-containing protein 1". It is hypotheQuantityd that the START domain forms a pocket in StAR that binds single cholesterol molecµLes for delivery to P450scc. Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calcµLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).