ELISA Squalene monooxygenase (SQLE)

Reactivity: (Homo sapiens) UniProt:Q14534 Abbreviation:SQLE Alternative Names:FLJ30795; squalene monooxygenase Application:ELISA Range:0.312-20 ng/mL Sensitivity:0.114 ng/mL Intra-AssayCV:?4.4% Inter-AssayCV:?7.1% Recovery:0.95 Sample Type:Serum, Plasma, Other biological fluids Detection Method:Sandwich Analysis Method??:Quantitive Test principle:This assay employs a two-site sandwich ELISA to quantitate SQLE in samples. An antibody specific for SQLE has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySQLE present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjµgated antibody specific for SQLE is added to the wells. After washing, Streptavidin conjµgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SQLE bound in the initial step. The color development is stopped and the intensity of the color is measured. Product Overview:Squalene epoxidase (EC 1.14.99.7) catalyzes the first oxygenation step in sterol biosynthesis and is thoµght to be one of the rate-limiting enzymes in this pathway. Squalene epoxidase (SE) is a key flavin adenine dinucleotide (FAD)-dependent enzyme of ergosterol and cholesterol biosynthetic pathways and an attractive potential target for drµgs used to inhibit the growth of pathogenic fungi or to lower cholesterol level. By PCR analysis of a /rodent somatic cell hybrid mapping panel, Nagai et al. (1997) demonstrated that the SQLE gene maps to chromosome 8. The localization was refined by PCR analysis of a radiation hybrid panel. The resµLts showed that SQLE is most tightly linked to D8S508, which is reported to be located at 8q24.13-qter. The authors used fluorescence in situ hybridization to map SQLE to 8q24.1. Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calcµLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).