New
and Emerging Technologies: Improved Laboratory and On-Site
Detection of OIE List A Viruses in Animals and Animal Products
Detection of OIE List A Viruses in Animals and Animal Products
Produced by Macom
Sweden 2005-2008
Last updated: June 9 2008
Sweden 2005-2008
Last updated: June 9 2008
Innovation Aspects
This
proposal will develop simple robust pen-side tests and novel nucleic-acid-based
and antigen-antibody detection methods for the improved diagnosis of OIE
List A diseases. This combination, within a single project is, in itself,
innovative.
By addressing the recommendations of the Scientific Committee on Animal Health and Animal Welfare (SCAHAW, see in B.1) the proposal has a wide range of innovative aspects. Improved sample enrichment procedures will be developed, which will increase the detection capacity. Amplification without thermocycling will be applicable to “front line” diagnosis and novel nucleic acid detection methods, like padlock probes (unknown to date in veterinary medicine) will be developed. The development of rapid readout formats will open innovative aspects towards the introduction of microarrays for routine diagnostic use in veterinary virology. Novel PriProET real-time PCR assays will provide robotic, high throughput, complex diagnosis of OIE List A viruses within a very short time. The real-time PCR techniques will be adapted to portable PCR equipment, in order to further strengthen the “first line” diagnosis in field laboratories, close to the outbreaks. The research to increase the sensitivity of Ag-ELISA systems will yield very practical tools for rapid diagnosis in simply equipped laboratories and the simple dip-stick tests for virus or viral antibody detection are novel in the context of veterinary diagnostic virology. For selected viruses also antibody-ELISAs will be developed. The complex diagnostic arsenal will be standardised across the EU.
By addressing the recommendations of the Scientific Committee on Animal Health and Animal Welfare (SCAHAW, see in B.1) the proposal has a wide range of innovative aspects. Improved sample enrichment procedures will be developed, which will increase the detection capacity. Amplification without thermocycling will be applicable to “front line” diagnosis and novel nucleic acid detection methods, like padlock probes (unknown to date in veterinary medicine) will be developed. The development of rapid readout formats will open innovative aspects towards the introduction of microarrays for routine diagnostic use in veterinary virology. Novel PriProET real-time PCR assays will provide robotic, high throughput, complex diagnosis of OIE List A viruses within a very short time. The real-time PCR techniques will be adapted to portable PCR equipment, in order to further strengthen the “first line” diagnosis in field laboratories, close to the outbreaks. The research to increase the sensitivity of Ag-ELISA systems will yield very practical tools for rapid diagnosis in simply equipped laboratories and the simple dip-stick tests for virus or viral antibody detection are novel in the context of veterinary diagnostic virology. For selected viruses also antibody-ELISAs will be developed. The complex diagnostic arsenal will be standardised across the EU.
Thus,
the research on the development of such simple assays will provide novelty
and practical applicability in the “first line” diagnostic
work and will combine this with a wide range of innovative issues, both
in nucleic-acid-based diagnosis and in antigen-antibody detection. The
innovative nature of the planned research is shown by the fact that the
members of the LAB-ON-SITE consortium are publishing their results in journals
like Nature Biotechnology and Nucleic Acids Research (Hardenbol et al.,
2003; Banér et al., 2003), Archives of Virology (Rasmussen et al.,
2003), Expert Review of Molecular Diagnostics (Belák and Thorén,
2001) and many other peer-reviewed high standard international journals.
The composition of this new consortium is, in itself, innovative and the interactions, exchanges and transfer of procedures anticipated through the formulation of the multidisciplinary workpackages within the project add a new dimension to this innovation. State of the art technologies developed for studies within one discipline will be transferred and applied to other disciplines; veterinarians and molecular biologists will be directly interfacing with, and applying expertise from experts in classical virology, molecular virology, molecular epizootiology and immunology. It is anticipated that this unique blend and exchange of expertise within the consortium working on OIE List A diseases will generate a better and much needed, arsenal of powerful, modern, rapid and highly sensitive diagnostic methods for veterinary virology.
The composition of this new consortium is, in itself, innovative and the interactions, exchanges and transfer of procedures anticipated through the formulation of the multidisciplinary workpackages within the project add a new dimension to this innovation. State of the art technologies developed for studies within one discipline will be transferred and applied to other disciplines; veterinarians and molecular biologists will be directly interfacing with, and applying expertise from experts in classical virology, molecular virology, molecular epizootiology and immunology. It is anticipated that this unique blend and exchange of expertise within the consortium working on OIE List A diseases will generate a better and much needed, arsenal of powerful, modern, rapid and highly sensitive diagnostic methods for veterinary virology.